Effects of the Hippocampal Deep Brain Stimulation on Cortical Epileptic Discharges in Penicillin – Induced Epilepsy Model in Rats Ratlarda Penisilinle İndüklenmiş Deneysel Epilepsi Modelinde Hipokampal Derin Beyin Stimülasyonun Kortikal İktal Aktivite Üzerine Etkileri
نویسنده
چکیده
AIm: Experimental and clinical studies have revealed that hippocampal DBS can control epileptic activity, but the mechanism of action is obscure and optimal stimulation parameters are not clearly defined. The aim was to evaluate the effects of high frequency hippocampal stimulation on cortical epileptic activity in penicillin-induced epilepsy model. mAterIAl and methOds: Twenty-five Sprague-Dawley rats were implanted DBS electrodes. In group-1 (n=10) hippocampal DBS was off and in the group-2 (n=10) hippocampal DBS was on (185 Hz, 0.5V, 1V, 2V, and 5V for 60 sec) following penicillin G injection intracortically. In the control group hippocampal DBS was on following 8μl saline injection intracortically. EEG recordings were obtained before and 15 minutes following penicillin-G injection, and at 10th minutes following each stimulus for analysis in terms of frequency, amplitude, and power spectrum. results: High frequency hippocampal DBS suppressed the acute penicillin-induced cortical epileptic activity independent from stimulus intensity. In the control group, hippocampal stimulation alone lead only to diffuse slowing of cerebral bioelectrical activity at 5V stimulation. COnClusIOn: Our results revealed that continuous high frequency stimulation of the hippocampus suppressed acute cortical epileptic activity effectively without causing secondary epileptic discharges. These results are important in terms of defining the optimal parameters of hippocampal DBS in patients with epilepsy. KeywOrds: Penicillin-induced, Epilepsy, Hippocampus, Deep brain stimulation, Parameter
منابع مشابه
The Effects of Pregabalin on Cerebral Cortical Oxidative Stress of Rats on Pentylenetetrazole Induced Epileptic Seizure Sıçanlarda Pentilentetrazol ile Oluşturulan Epileptik Nöbet Modelinde Pregabalinin Beyin Korteksinde Oksidatif Stres Üzerine Etkileri
Methods: Forty male Wistar rats were randomly divided into four equal groups. The first group was used as a control group. The second group received a single dose administration of PTZ. Third and fourth groups were given, via gastric gavage, doses of 100 mg/kg body weight/day of PGB, divided into two, for two days. Seizure is obtained by applying intraperitoneally (ip) 50 mg/kg body weight of P...
متن کاملControl of epileptic seizures by electrical low frequency deep brain stimulation: A review of probable mechanisms
Epilepsy is the most common neurological disease with no definitive method in treatment. Notably, the main way to treat and control epileptic seizures is drug therapy. However, about 20-30% of patients with epilepsy are drug resistant and require other therapeutic manners. Deep brain stimulation is a new therapeutic strategy for these patients. Conspicuously, there are no clear answers for basi...
متن کاملThe Neuroprotective Effects of Long-Term Repetitive Transcranial Magnetic Stimulation on the Cortical Spreading Depression-induced Damages in Rat’s Brain
Introduction: Cortical Spreading Depression (CSD) is a propagating wave of neural and glial cell depolarization with important role in several clinical disorders. Repetitive Transcranial Magnetic Stimulation (rTMS) is a potential tool with preventive treatment effects in psychiatric and neuronal disorders. In this paper, we study the effects of rTMS on CSD by using behavioral and histological a...
متن کاملProconvulsive effect of hydrochlorothiazide in an in vitro rat seizure model
Objective(s):Protective effects of diuretics, particularly of hydrochlorothiazide (HCT), for the development of seizure attacksepilepsy have been described in vivo. However, itsthe mechanism of action of HCT is unknownneeds to be elucidated. Materials and Methods: Extracellular field potentials were recorded from the CA1- and CA3-subfields of the hippocampus of rats. Epileptiform discharges wer...
متن کاملElectrophysiological characteristics of hippocampal CA1 neurons after spreading depression-triggered epileptic activity in brain slices
Introduction: A close link between spreading depression (SD) and several neurological diseases such as epilepsy could be demonstrated in many experimental studies. Epilepsy is among the most common brain disorders. Despite a large number of investigations, its mechanisms have not been yet well elucidated. Hippocampus is one of the important structures involved in seizures. The aim of this st...
متن کامل